| 质粒名称: | pLX304 CMV OMM-FKBP-V5-sTurboID (N) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 153006 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pLX304 |
| 空载体大小: | 7668 bp |
| 完整质粒大小: | 8445 bp |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | 杀稻瘟菌素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | OMM (Tom20)-sTurboID (N) (L73) |
| 插入大小: | 777 bp |
| 启动子: | CMV |
| 标签/融合蛋白: |
FKBP
V5
|
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | CGCAAATGGGCGGTAGGCGTG |
|
文献引用方法 |
|
| 材料和方法部分: | pLX304 CMV OMM-FKBP-V5-sTurboID (N) was a gift from Alice Ting (Addgene plasmid # 153006 ; http://n2t.net/addgene:153006 ; RRID:Addgene_153006) |
| 参考文献部分: | Split-TurboID enables contact-dependent proximity labeling in cells. Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak C, Rhee HW, Lee IK, Carr SA, Ting AY. Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 10.1073/pnas.1919528117. 10.1073/pnas.1919528117 PubMed 32424107 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLX304 CMV OMM-FKBP-V5-sTurboID (N) 8445 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses split-TurboID N-terminal fragment targeted to outer
mitochondrial membrane.
ACCESSION .
VERSION .
KEYWORDS pLX304 CMV OMM-FKBP-V5-sTurboID (N)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8445)
AUTHORS Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak
C, Rhee HW, Lee IK, Carr SA, Ting AY
TITLE Split-TurboID enables contact-dependent proximity labeling in cells.
JOURNAL Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi:
10.1073/pnas.1919528117.
PUBMED 32424107
REFERENCE 2 (bases 1 to 8445)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8445
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 65..368
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 369..572
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 523..543
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 595..619
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 769..1089
/codon_start=1
/product="human FK506-binding protein FKBP12"
/label=FKBP
/translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
KLE"
CDS 1120..1161
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 1452..2040
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1505..1525)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(1923..1934)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 2112..2345
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2423..2544
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2460..2479)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2514..2533
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 2584..2719
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2646..2665
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2739..2758)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2740..2758)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2768..2785)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2768..2784)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2777..2799)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2926..3381
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3013..3032)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3223..3244
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3407..3511
/gene="bla"
/label=AmpR promoter
CDS 3512..4372
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3730..3749)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4543..5131
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5032..5051
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5285..5302
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5419..5440
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5455..5485
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5493..5509
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5498..5520
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5517..5533
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5517..5533
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 5552..5572
/label=T3
/note="T3 promoter, forward primer"
promoter 5554..5572
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 5600..5826
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 5827..6007
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6054..6179
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6672..6905
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7090..7134
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 7309..7809
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 7728..7746
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 7821..8216
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 8276..8393
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg tgggtcggaa cagcgccatc
661 gccgcgggcg tgtgcggtgc cctcttcata gggtactgca tctactttga ccgcaaaaga
721 cgaagtgacc ccaacttcgg atccggagca agtggaagtg ctggtggtgg agtgcaggtg
781 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
841 cactacaccg ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag
901 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
961 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
1021 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
1081 aaactggaag ggagtggcag cggaggtagt gggagcggtg gcaagcccat ccccaacccc
1141 ctgctgggcc tggacagcac caagggctcg ggctcgacct cgggctcggg caccggtaaa
1201 gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt ccatagtggc
1261 gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca cattcagact
1321 ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc tctgcccgag
1381 cctatcccgc tgctgaacgc taaacagatt ctgtaataag ctagcgctaa ccggtggcgc
1441 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
1501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
1561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
1621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
1681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
1741 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
1801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
1861 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
1921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
1981 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
2041 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
2101 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
2161 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
2221 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
2281 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
2341 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
2401 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
2461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
2521 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
2581 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
2641 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
2701 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
2761 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
2821 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
2881 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
2941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
3001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
3061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
3121 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
3181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
3241 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
3301 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
3361 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
3421 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
3481 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
3541 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
3601 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
3661 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
3721 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
3781 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
3841 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
3901 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
3961 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
4021 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
4081 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
4141 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
4201 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
4261 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
4321 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
4381 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
4441 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
4501 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
4561 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
4621 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
4681 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
4741 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
4801 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
4861 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
4921 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
4981 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
5041 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
5101 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
5161 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
5221 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
5281 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
5341 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
5401 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
5461 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
5521 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
5581 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
5641 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
5701 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
5761 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
5821 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
5881 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
5941 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
6001 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
6061 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
6121 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
6181 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
6241 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
6301 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
6361 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
6421 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
6481 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
6541 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
6601 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
6661 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
6721 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
6781 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
6841 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
6901 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
6961 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
7021 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
7081 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
7141 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
7201 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
7261 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
7321 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
7381 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
7441 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
7501 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
7561 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
7621 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
7681 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
7741 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
7801 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
7861 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
7921 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
7981 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
8041 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
8101 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
8161 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
8221 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
8281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
8341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
8401 attacaggga cagcagagat ccactttggc gccggctcga ggggg
//
